FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3453.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3453.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215889
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCTCGTAT19761191.5336121803334TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAAACTATCTCGTAT9260.4289241230447128TruSeq Adapter, Index 7 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6080.28162620605959543No Hit
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCTCGTA4080.18898600669788643TruSeq Adapter, Index 7 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGCCTATCTCGTAT3750.17370037380320444TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCGCGTAT3730.17277397180958734TruSeq Adapter, Index 7 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCTCGTATG3550.1644363538670335TruSeq Adapter, Index 7 (97% over 36bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCTCGTA3480.16119394688937372TruSeq Adapter, Index 7 (97% over 35bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCTCGTA3460.16026754489575662TruSeq Adapter, Index 7 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACGATCTCGTAT3190.1477611179819259TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATATCGTAT2990.138497098045755TruSeq Adapter, Index 7 (97% over 37bp)
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCTCGTAT2620.12135866116383881TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCTCCTAT2590.11996905817341318TruSeq Adapter, Index 7 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCTCGTATGCC2180.10097781730426285TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCT550.070.0078857
ACACGGC150.00222145870.0078813
CTATATC500.070.0078840
CATGAAA600.070.0078862
CACACGG150.00222145870.0078812
TATATCG500.070.0078841
CTTTAAA600.070.0078862
GCCTATC550.070.0078838
TATGCTT550.070.0078858
GTCGTCT252.8312825E-670.0078854
TCGGCTT150.00222145870.0078858
TAAGCCG150.00222145870.0078848
CACGTCA150.00222145870.0078814
TATCGTA500.070.0078843
GTATTCT252.8312825E-670.0078854
GTCTGCT150.00222145870.0078857
CCGTCGT301.01954356E-770.0078852
TATGACG150.00222145870.0078848
TTCCCTG150.00222145870.0078850
GCTATCT150.00222145870.0078839
Produced by FastQC (version 0.11.5)