FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3429.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3429.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241416
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTCGTAT22207591.98851774530272TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATATCGTAT10170.42126453921861023TruSeq Adapter, Index 22 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6220.2576465520098088No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTCGTATG4480.1855717930874507TruSeq Adapter, Index 22 (97% over 36bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTCGTA4230.17521622427676709TruSeq Adapter, Index 22 (97% over 35bp)
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTCGTAT3800.15740464592239123TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAACTAATCTCGTAT3650.15119130463598104TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTATCTCGTATG3570.14787752261656228TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTCGTA3500.14497796334957086TruSeq Adapter, Index 22 (96% over 33bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTCGTA3410.14124995857772477TruSeq Adapter, Index 2 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACGAATCTCGTAT3020.12509527123305828TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAACTAATCTCGTAT2780.115153925174802TruSeq Adapter, Index 1 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTCGTATGCC2710.11225436590781059TruSeq Adapter, Index 22 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAAATAATCTCGTAT2420.10024190608741758TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAC252.833247E-670.0039433
CGCGACT252.833247E-670.0039434
CTCGAAT150.002222273770.0039444
ACAGCAA401.3460522E-1070.0039432
CTTCAGC150.002222273770.0039456
AACAATC207.913748E-570.0039437
CCAATCA207.913748E-570.0039426
CCGGAAC207.913748E-570.0039433
AGCTCGT252.833247E-670.0039442
GTCACCC150.002222273770.0039429
GTCACAG401.3460522E-1070.0039429
CAGCAAC301.02045306E-770.0039433
GCGACTA207.913748E-570.0039435
TGACCGC150.002222273770.0039430
CCAGTGA150.002222273770.0039426
TGACTCC150.002222273770.0039420
AACGAAT401.3460522E-1070.0039437
CCAGTAC401.3460522E-1070.0039426
CAACTTA150.002222273770.0039436
AACTGAT252.833247E-670.0039437
Produced by FastQC (version 0.11.5)