FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3424.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3424.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986921
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTCGTAT22059411.102303513828684TruSeq Adapter, Index 2 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG234900.068.09848
GTATGCC235400.067.98138447
CGTATGC235800.067.8757846
TCGTATG236200.067.6998645
ATGCCGT236650.067.5682449
CTCGTAT237800.067.1526644
CTGCTTG238500.067.00178559
TGCCGTC238750.066.9902750
GCCGTCT240200.066.558451
TGCTTGA240350.066.4827160
TCTCGTA240450.066.3106943
ATCTCGT240800.066.2143142
CCGTCTT242050.066.0769552
GCTTGAA242300.066.00545561
TCTGCTT243100.065.7771558
AATCTCG241650.065.666141
CTTCTGC247550.064.5947456
TTCTGCT249600.064.0221457
AAATCTC248550.063.70072640
GAAATCT248850.063.37397839
Produced by FastQC (version 0.11.5)