FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3423.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3423.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886279
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAAGGTGATCTCGTAT40289421.35919447759319TruSeq Adapter, Index 19 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC423950.068.8056647
CGTATGC424300.068.7782546
TATGCCG424450.068.7264348
TCGTATG425200.068.6244445
ATGCCGT425950.068.4761949
CTCGTAT426100.068.36449444
TGCCGTC426700.068.3512550
CTGCTTG427800.068.2118559
GCCGTCT427650.068.1884751
TGCTTGA428650.068.1174960
TCTCGTA427850.068.04397643
GATCTCG426400.068.0291241
ATCTCGT427950.067.8972242
TCTGCTT430300.067.8526258
CCGTCTT429700.067.8468652
GGTGATC423600.067.7958838
GTGATCT425250.067.63158439
GCTTGAA431750.067.6157861
TGATCTC427400.067.5024840
GTAAGGT426850.067.3477934
Produced by FastQC (version 0.11.5)