FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2478929
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCACCATCTCGTAT33880113.667232905823443TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC361300.068.0785346
TATGCCG361350.068.0455548
GTATGCC361400.068.02370547
ATGCCGT362900.067.74245549
TCGTATG363950.067.563645
CTCGTAT363750.067.3684844
TGCCGTC365000.067.36365550
CTGCTTG365250.067.2751559
GCCGTCT366150.067.1452351
TGCTTGA367600.066.8954560
CCGTCTT368350.066.7550552
TCTCGTA367000.066.7432843
ATCTCGT368050.066.5148342
TCTGCTT369650.066.4675758
GCTTGAA371200.066.3517361
CATCTCG367200.066.3365641
ACCATCT368550.065.7218939
CCATCTC370650.065.6409140
CTTCTGC376000.065.3849256
TTCTGCT377050.065.165757
Produced by FastQC (version 0.11.5)