FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3417.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3417.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1437892
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCTCGTAT23572416.393720807960545TruSeq Adapter, Index 9 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG251950.068.0830548
CGTATGC252250.068.0690946
TCGTATG252700.067.9201745
GTATGCC252800.067.9071547
ATGCCGT253100.067.7460549
TGCCGTC253400.067.6567450
CTCGTAT253650.067.50490644
GCCGTCT254050.067.47455651
CTGCTTG254250.067.4167959
TGCTTGA255850.067.0862860
CCGTCTT255600.067.0653852
TCTCGTA255200.067.0263443
ATCTCGT256350.066.6960342
TCTGCTT257150.066.65649458
CATCTCG255900.066.61046641
ACATCTC254750.066.59283440
GCTTGAA257900.066.5824861
GCACATC254200.066.4248638
GGCACAT254200.066.4225537
CACATCT254900.066.40723439
Produced by FastQC (version 0.11.5)