FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3416.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3416.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520843
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGAGATATCTCGTAT32020621.054507269981187TruSeq Adapter, Index 1 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC341400.068.377546
GTATGCC341450.068.3594847
ATGCCGT341950.068.3027249
TATGCCG341700.068.30147648
TCGTATG342750.068.1388245
CTCGTAT342850.068.0781144
TGCTTGA343750.067.924760
TGCCGTC343750.067.924750
CTGCTTG344050.067.85306559
GCCGTCT344150.067.8254151
TCTCGTA344700.067.6315143
GCTTGAA346450.067.4155461
CCGTCTT346500.067.4080352
TCTGCTT346850.067.3478958
ATCTCGT346200.067.3283642
TATCTCG345100.067.30971541
ATATCTC346400.066.9481740
CTTCTGC350950.066.63309556
GATATCT347900.066.5287339
AGATATC348350.066.4026138
Produced by FastQC (version 0.11.5)