FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3413.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3413.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259924
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGCTAGATCTCGTAT51516222.795545336922835TruSeq Adapter, Index 12 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG541350.068.3361848
GTATGCC541450.068.3285147
CGTATGC542000.068.2675646
ATGCCGT542550.068.19349
TCGTATG542550.068.1903945
CTGCTTG544050.068.0060759
TGCCGTC544250.067.9667150
TGCTTGA544700.067.9476260
CTCGTAT543350.067.9225244
GCCGTCT544950.067.87791451
CCGTCTT546950.067.648952
TCTCGTA545300.067.6139543
GCTTGAA547400.067.6060861
ATCTCGT546650.067.4021642
TCTGCTT550100.067.2772458
GATCTCG546650.067.235741
TAGATCT546200.067.07027439
CTTCTGC552900.066.9287256
AGATCTC548600.066.9235840
TTCTGCT554300.066.78641557
Produced by FastQC (version 0.11.5)