FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3408.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3408.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301124
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCTCGTAT42340212.825995024724913TruSeq Adapter, Index 11 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG449050.068.2265248
CGTATGC449200.068.1840246
GTATGCC449450.068.16373447
ATGCCGT449750.068.1031749
TCGTATG450950.067.90594545
CTCGTAT452450.067.6266844
TGCCGTC453200.067.60120450
GCCGTCT453800.067.50513551
CTGCTTG454400.067.3944759
TGCTTGA456550.067.0893860
CCGTCTT457950.066.8724952
TCTCGTA458000.066.7689743
TCTGCTT461250.066.403258
ATCTCGT461800.066.1948142
GCTTGAA463250.066.15027661
CATCTCG462100.066.0457941
ACATCTC463200.065.689540
TAACATC462550.065.629538
CTTCTGC467950.065.4778656
TTCTGCT468600.065.3561757
Produced by FastQC (version 0.11.5)