FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3404.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3404.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3911016
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCTCGTAT3849769.843375736637231TruSeq Adapter, Index 8 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG410200.068.13360648
CGTATGC410650.068.08694546
GTATGCC410750.068.0465547
TCGTATG412450.067.7567245
ATGCCGT413500.067.6102449
TGCCGTC415500.067.2705350
CTCGTAT415200.067.2160844
GCCGTCT417750.066.89316651
CTGCTTG419850.066.5868259
TCTCGTA419650.066.4282643
TGCTTGA421350.066.3638460
CCGTCTT422900.066.11166452
GATCTCG421450.066.0125241
ATCTCGT421800.065.9992342
CTAGGAG418600.065.8265835
GCTTGAA425650.065.6942661
AGATCTC423500.065.6160940
GAGATCT424750.065.27466639
TCTGCTT428650.065.2214958
TAGGAGA423250.065.1670436
Produced by FastQC (version 0.11.5)