FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3402.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3402.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161072
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGTAT35031430.171600038585034TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG377700.068.8480348
GTATGCC378250.068.76642647
CGTATGC378400.068.76099446
ATGCCGT378400.068.6929249
TCGTATG378850.068.6793245
CTCGTAT378500.068.54568544
TGCCGTC379100.068.5258250
GCCGTCT379400.068.4808651
CTGCTTG379550.068.4726159
TGCTTGA380000.068.44089560
CCGTCTT379900.068.3815152
TCTGCTT380750.068.2689458
TCTCGTA380400.068.1665243
GCTTGAA381950.068.11896561
ATCTCGT381100.067.9924642
GATCTCG380250.067.8470141
CTTCTGC383300.067.7811656
TTCTGCT384050.067.6641157
CGTCTTC384000.067.6331653
GGATCTC380150.067.625540
Produced by FastQC (version 0.11.5)