FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3396.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3396.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400351
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAACATCTCGTAT23752116.961533215600948TruSeq Adapter, Index 11 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG255200.068.244248
CGTATGC255550.068.1756946
GTATGCC255700.068.135747
TCGTATG256000.068.01726545
ATGCCGT256050.067.9614449
CTGCTTG256800.067.6923859
CTCGTAT256800.067.6394144
TGCCGTC257300.067.5928750
TGCTTGA257950.067.3833760
GCCGTCT258550.067.2955751
CCGTCTT258900.067.2181252
TCTCGTA258300.067.1788743
TCTGCTT259300.067.1072458
TAACATC256300.066.9086838
GCTTGAA260550.066.72201561
ATCTCGT260800.066.5506942
ACATCTC258750.066.5074640
CTTCTGC262150.066.39339456
TTAACAT257550.066.3824937
TTCTGCT262600.066.34627557
Produced by FastQC (version 0.11.5)