Basic Statistics
Measure | Value |
---|---|
Filename | HMT3KBGX7_n01_QG3389.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1651192 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATCTCGTAT | 178469 | 10.808494711699185 | TruSeq Adapter, Index 1 (97% over 39bp) |
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT | 2623 | 0.15885493631267594 | No Hit |
CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCT | 2368 | 0.1434115475365675 | No Hit |
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG | 1923 | 0.1164613200645352 | No Hit |
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC | 1777 | 0.10761922296135155 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTATGCC | 18880 | 0.0 | 68.167114 | 47 |
TATGCCG | 18895 | 0.0 | 68.10066 | 48 |
CGTATGC | 18935 | 0.0 | 67.96705 | 46 |
TCGTATG | 18945 | 0.0 | 67.949646 | 45 |
ATGCCGT | 18940 | 0.0 | 67.92244 | 49 |
TGCCGTC | 19110 | 0.0 | 67.34264 | 50 |
CTGCTTG | 19120 | 0.0 | 67.31554 | 59 |
CTCGTAT | 19110 | 0.0 | 67.27138 | 44 |
GCCGTCT | 19245 | 0.0 | 66.943016 | 51 |
TGCTTGA | 19250 | 0.0 | 66.8771 | 60 |
TCTCGTA | 19235 | 0.0 | 66.75941 | 43 |
ATCTCGT | 19320 | 0.0 | 66.4818 | 42 |
GCCATCT | 19080 | 0.0 | 66.37854 | 39 |
CCGTCTT | 19400 | 0.0 | 66.37206 | 52 |
ACGCCAT | 19225 | 0.0 | 65.97092 | 37 |
TCTGCTT | 19535 | 0.0 | 65.83373 | 58 |
CGCCATC | 19260 | 0.0 | 65.75818 | 38 |
TAACGCC | 19330 | 0.0 | 65.727165 | 35 |
CATCTCG | 19440 | 0.0 | 65.67535 | 41 |
GCTTGAA | 19645 | 0.0 | 65.61951 | 61 |