FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3389.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3389.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651192
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATCTCGTAT17846910.808494711699185TruSeq Adapter, Index 1 (97% over 39bp)
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT26230.15885493631267594No Hit
CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCT23680.1434115475365675No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG19230.1164613200645352No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC17770.10761922296135155No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC188800.068.16711447
TATGCCG188950.068.1006648
CGTATGC189350.067.9670546
TCGTATG189450.067.94964645
ATGCCGT189400.067.9224449
TGCCGTC191100.067.3426450
CTGCTTG191200.067.3155459
CTCGTAT191100.067.2713844
GCCGTCT192450.066.94301651
TGCTTGA192500.066.877160
TCTCGTA192350.066.7594143
ATCTCGT193200.066.481842
GCCATCT190800.066.3785439
CCGTCTT194000.066.3720652
ACGCCAT192250.065.9709237
TCTGCTT195350.065.8337358
CGCCATC192600.065.7581838
TAACGCC193300.065.72716535
CATCTCG194400.065.6753541
GCTTGAA196450.065.6195161