FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3380.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3380.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365274
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGATTGATCTCGTAT25774710.89713073411368TruSeq Adapter, Index 20 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC277800.068.3498847
TATGCCG277850.068.3293248
CGTATGC278750.068.1406146
ATGCCGT279300.067.9706849
TCGTATG279400.067.9666845
CTCGTAT280100.067.60940644
TGCCGTC280750.067.5865150
GCCGTCT281150.067.5056651
CTGCTTG283200.067.0441759
TCTCGTA281900.067.0301443
TGCTTGA283850.066.9328960
GATCTCG280200.066.8705141
CCGTCTT284200.066.7702952
ATCTCGT283100.066.58530442
TCTGCTT287950.066.026158
GCTTGAA288700.065.8476161
TGATCTC284250.065.7809140
ATTGATC285900.065.206838
TTGATCT288700.064.5730239
CGATTGA288800.064.5238636
Produced by FastQC (version 0.11.5)