FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3378.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3378.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881465
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCATTGCATCTCGTAT30963816.45728195847385TruSeq Adapter, Index 6 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG329000.068.3176748
GTATGCC329250.068.23208647
CGTATGC329500.068.2085646
TCGTATG330150.068.06341645
ATGCCGT330400.068.019449
CTCGTAT330200.067.86233544
TGCCGTC331600.067.74518650
GCCGTCT332150.067.63351
TGCTTGA333400.067.4352660
CTGCTTG333450.067.3972459
TCTCGTA332800.067.34267443
CCGTCTT334100.067.2382652
CATCTCG332650.067.0153441
ATCTCGT334500.066.9690442
TGCATCT331600.066.87749539
GCATCTC332500.066.85435540
GCTTGAA336650.066.8326861
TCTGCTT336450.066.7962858
TTGCATC333600.066.3506738
CTTCTGC339300.066.1939556
Produced by FastQC (version 0.11.5)