FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3374.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3374.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2169389
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCTCGTAT37826817.43661464126535TruSeq Adapter, Index 16 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG402950.068.2455348
CGTATGC403350.068.20312546
TCGTATG403600.068.1608845
GTATGCC403600.068.14982647
ATGCCGT404300.068.038149
CTCGTAT405450.067.70470444
CTGCTTG406200.067.6728459
TGCTTGA406500.067.66987660
GCCGTCT406800.067.63637551
TGCCGTC407000.067.59141550
CCGTCTT408650.067.3145952
TCTCGTA408400.067.1829243
GCTTGAA410450.067.02717661
ATCTCGT409550.067.0028242
TCTGCTT410950.066.90069658
TATCTCG409200.066.8393141
TTATCTC408900.066.6401340
CTTATCT409100.066.4636639
CTTCTGC415400.066.21619456
GTGCTTA408800.066.0421436
Produced by FastQC (version 0.11.5)