FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3369.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3369.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038106
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGTAT33697516.53373278916798TruSeq Adapter, Index 18 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC360350.068.2000546
TATGCCG360050.068.1824648
GTATGCC360300.068.1545847
ATGCCGT360750.068.0504749
TCGTATG361350.067.9321645
TGCCGTC362000.067.85750650
CTGCTTG361300.067.8563559
CTCGTAT360850.067.7175944
GCCGTCT363200.067.5851151
TGCTTGA363650.067.47063460
TCTCGTA363150.067.2308943
CCGTCTT365100.067.2238152
TCTGCTT366400.066.951758
GCTTGAA367500.066.8606861
ATCTCGT366750.066.5614142
AATCTCG366200.066.4415641
CTTCTGC371350.066.0891556
TTCTGCT373100.065.7604157
GAATCTC372550.065.1743740
TTGAATC370750.065.1164138
Produced by FastQC (version 0.11.5)