FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3361.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3361.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2379003
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGTAT45015718.92208626891181TruSeq Adapter, Index 22 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC479400.068.5431447
CGTATGC480000.068.4823146
TATGCCG480150.068.4535348
ATGCCGT480950.068.3512649
TCGTATG481250.068.265245
TGCCGTC481950.068.2151750
CTCGTAT480900.068.1270944
GCCGTCT482800.068.1124451
CTGCTTG482350.068.1092359
TGCTTGA483700.067.9453360
TCTCGTA481850.067.84608543
CCGTCTT486500.067.6088252
TCTGCTT486400.067.5421158
ATCTCGT484100.067.4787242
GCTTGAA487350.067.4723661
GATCTCG483950.067.38676541
TACGATC481150.067.2842938
ACGATCT483100.067.1952339
CGATCTC484150.067.1261940
CTTCTGC491700.066.8880956
Produced by FastQC (version 0.11.5)