FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3348.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3348.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604315
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACCACAATCTCGTAT28213717.586134892461892TruSeq Adapter, Index 18 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG303050.068.262648
GTATGCC303200.068.2224547
CGTATGC303350.068.2096646
TCGTATG303400.068.15015445
ATGCCGT303750.068.0707149
CTCGTAT303600.067.89776644
TGCCGTC304700.067.84750
CTGCTTG304750.067.7805559
GCCGTCT305750.067.6046551
TGCTTGA306000.067.5316760
TCTCGTA305550.067.3613643
GCTTGAA308650.067.019961
ATCTCGT307500.066.9434842
CCGTCTT308850.066.9034252
TCTGCTT309600.066.73926558
AATCTCG307300.066.71580541
CAATCTC306300.066.6687440
ACAATCT306100.066.6021339
CACAATC305900.066.4397338
CTTCTGC311400.066.33308456
Produced by FastQC (version 0.11.5)