FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3338.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3338.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468179
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACACAGATCTCGTAT1208548.231557596178668TruSeq Adapter, Index 23 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC132750.067.81048647
TATGCCG133100.067.6870748
CGTATGC133200.067.581446
TCGTATG133900.067.2803745
ATGCCGT133800.067.2613549
TGCCGTC134950.066.7186550
CTGCTTG135600.066.3920559
CTCGTAT135450.066.3812644
GCCGTCT135900.066.25452451
TGCTTGA137000.065.7624560
CCGTCTT137300.065.55344452
GATCTCG136000.065.4694441
TCTCGTA137300.065.4613443
ATCTCGT136950.065.42419442
CAGATCT135600.065.2967739
TCTGCTT139150.064.7781458
GCTTGAA140100.064.40726561
AGATCTC137900.064.3853540
AACACAG138450.063.9107835
ACACAGA138600.063.73842636
Produced by FastQC (version 0.11.5)