FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3336.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3336.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2748969
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACGCTAATCTCGTAT2211898.046252977025205TruSeq Adapter, Index 4 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG238850.067.9097548
GTATGCC239050.067.8467647
CGTATGC239550.067.73067546
TCGTATG240950.067.3383645
ATGCCGT241200.067.21068649
CTGCTTG242600.066.92383659
CTCGTAT242000.066.7569444
TGCCGTC244200.066.38621550
GCTAATC240950.066.3167738
TGCTTGA245600.066.1561460
CGCTAAT242650.066.0564737
GCCGTCT245650.066.0525651
TAATCTC243350.065.9791240
ACGCTAA242550.065.8780736
TCTCGTA245800.065.6952443
GACGCTA244250.065.5079235
CTAATCT244700.065.41608439
ATCTCGT247100.065.3637742
CCGTCTT248700.065.2143552
TCTGCTT250150.064.88994658
Produced by FastQC (version 0.11.5)