FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3334.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3334.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783571
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCTCGTAT18065810.129005237245952TruSeq Adapter, Index 10 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC197350.067.7299246
GTATGCC197650.067.6429347
TATGCCG197800.067.5777348
ATGCCGT198400.067.449649
TCGTATG198550.067.3363245
TGCCGTC199900.066.8596750
GCCGTCT200200.066.7613651
CTCGTAT199450.066.7341544
CTGCTTG200250.066.7041159
TGCTTGA201000.066.5093360
TCTCGTA201350.066.1044343
GCAATCT199350.066.0930739
ATCTCGT201900.065.870542
CCGTCTT203000.065.8614552
AATCTCG201900.065.7318341
GCTTGAA204150.065.49840561
TACCTGC200950.065.4806934
CTGCAAT200850.065.4637537
TGCAATC200750.065.4403938
TCTGCTT204600.065.3219758
Produced by FastQC (version 0.11.5)