FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3333.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3333.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281737
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTAT1981578.684480288481977TruSeq Adapter, Index 22 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG211500.067.98579448
GTATGCC211550.067.9803147
CGTATGC211950.067.88206546
ATGCCGT212400.067.6647649
TCGTATG212900.067.5477745
TGCCGTC212750.067.50851450
CTCGTAT214700.067.0466644
CTGCTTG216450.066.33835659
GCCGTCT216250.066.33787551
TGCTTGA217050.066.2517460
TCTCGTA217100.066.2409943
CCGTCTT218750.065.6117352
ACAGCAT216750.065.5876137
ATCTCGT219000.065.58639542
TACAGCA217300.065.42303536
GCTTGAA221400.065.0621561
GCATCTC219600.065.0073340
TTACAGC218850.064.8825635
AGCATCT219650.064.833239
CAGCATC219150.064.8238
Produced by FastQC (version 0.11.5)