FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3331.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3331.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690162
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACATCTCGTAT21271312.585361639890142TruSeq Adapter, Index 15 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG223350.068.3699548
CGTATGC223450.068.3216746
GTATGCC223600.068.2935147
TCGTATG224000.068.1050345
ATGCCGT224500.068.0277849
TGCCGTC226200.067.50304450
GCCGTCT226250.067.4766551
CTCGTAT225700.067.3904744
TGCTTGA226950.067.31428560
CTGCTTG226750.067.313959
TCTCGTA227200.066.86655443
CCGTCTT228750.066.754552
ATCTCGT228150.066.6034642
GCTTGAA230150.066.3955261
TCTGCTT230350.066.261958
CATCTCG228450.066.1656141
ACACATC226950.066.0920938
CACATCT227500.066.08613639
ACATCTC228400.065.9636340
CTTCTGC233550.065.4497456
Produced by FastQC (version 0.11.5)