FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3330.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3330.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431792
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCCATATCTCGTAT28128311.566902103469376TruSeq Adapter, Index 16 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC302500.068.2190647
TATGCCG302550.068.2091948
CGTATGC303150.068.1060346
TCGTATG303400.068.04851545
ATGCCGT303350.068.0090349
CTCGTAT305300.067.5534644
TGCCGTC305750.067.5095450
GCCGTCT307100.067.1854851
CTGCTTG307750.067.0963159
TCTCGTA308400.066.8517243
TGCTTGA309700.066.74747560
CCGTCTT309550.066.6324852
CATATCT306900.066.5640339
TATCTCG308350.066.52206441
CCATATC307400.066.36468538
ATCTCGT311600.066.1624642
TCTGCTT312800.066.0368158
GCTTGAA313200.065.9792261
ATATCTC310750.065.87450440
TCCATAT309950.065.697237
Produced by FastQC (version 0.11.5)