FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3329.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3329.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2745812
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAGTCAATCTCGTAT27976310.188716488965742TruSeq Adapter, Index 18 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG297950.068.0412948
GTATGCC298600.067.9153847
CGTATGC298850.067.8665746
ATGCCGT299500.067.71622549
TCGTATG300300.067.5014645
TGCCGTC302150.067.1168550
CTCGTAT301500.066.8729544
CTGCTTG303350.066.87199459
TGCTTGA303850.066.7825660
GCCGTCT304200.066.6887851
TCTCGTA305650.065.91917443
AATCTCG305050.065.89728541
CCGTCTT308050.065.8780452
TCTGCTT309550.065.54272558
ATCTCGT307350.065.54198542
GCTTGAA309900.065.5126961
CAATCTC307950.065.1481540
GTCAATC306600.065.0138838
AGTCAAT311350.064.2917837
TCAATCT310650.064.2789339
Produced by FastQC (version 0.11.5)