FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3328.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3328.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589431
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCAGAGATCTCGTAT33081520.813423168416872TruSeq Adapter, Index 1 (97% over 36bp)
CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCT24410.15357697188490724No Hit
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT23690.14904704891247245No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG19700.12394372577356298No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC18250.11482096423185405No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG351450.068.6819448
CGTATGC352000.068.59582546
GTATGCC352450.068.50046547
ATGCCGT352500.068.4894349
TCGTATG352700.068.4519145
TGCCGTC353350.068.2949750
CTCGTAT353550.068.1982444
GCCGTCT354200.068.1628751
CTGCTTG354200.068.1465559
TGCTTGA355100.067.9927160
TCTCGTA354450.067.9658343
CCGTCTT356050.067.8185352
TCTGCTT356600.067.71735458
GATCTCG354800.067.6324441
ATCTCGT355600.067.6082442
GCTTGAA357450.067.57294561
AGATCTC356550.067.2100340
CTTCTGC360400.067.0367156
TTCTGCT361150.066.9071957
GAGATCT356650.066.8694539
Produced by FastQC (version 0.11.5)