FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3324.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3324.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2349584
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCTCGTAT2314779.851829089745248TruSeq Adapter, Index 6 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC246500.068.2140547
TATGCCG246400.068.1863648
CGTATGC246800.068.1013246
TCGTATG248350.067.6860545
ATGCCGT248200.067.6737349
CTCGTAT250100.067.1550344
TGCCGTC251100.066.89216650
CTGCTTG251050.066.85795659
GCCGTCT251300.066.8528551
TCTCGTA252350.066.52711543
CCGTCTT252900.066.5143752
TGCTTGA252900.066.40616660
CACGTTA250350.066.1821136
TATCTCG253050.066.1494541
ATCTCGT254100.066.0675342
GTTATCT251850.066.0463639
ACGTTAT251950.065.9104237
TTATCTC253700.065.7440740
CGTTATC252800.065.7303438
TCTGCTT256000.065.5802658
Produced by FastQC (version 0.11.5)