FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3323.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3323.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404323
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTATCTCGTAT1865267.757942672427956TruSeq Adapter, Index 6 (97% over 36bp)
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT31460.13084764401455212No Hit
CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCT24420.10156705234696004No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG197050.068.124948
GTATGCC196950.068.1211247
CGTATGC197550.067.92345446
ATGCCGT198600.067.59321649
TCGTATG198500.067.57934645
CTCGTAT199650.067.10103644
CTGCTTG200700.066.8831859
TGCCGTC200750.066.8762550
GCCGTCT202300.066.3839351
TGCTTGA202600.066.2331560
ACTATCT200300.065.8901239
TCTCGTA203650.065.78306643
CTATCTC201500.065.7061540
ATCTCGT204500.065.4925342
CCGTCTT205350.065.36384652
AACTATC201550.065.34119438
TATCTCG203600.065.3021141
CGAACTA202450.064.9683436
GCTTGAA206950.064.8930461
GAACTAT203350.064.7628137
Produced by FastQC (version 0.11.5)