FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3322.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3322.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797960
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCTCGTAT19825811.0268304077955TruSeq Adapter, Index 23 (97% over 38bp)
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT26390.14677745889786203No Hit
CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCT23060.12825646844201205No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG20150.11207145876437741No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC209200.067.9565447
CGTATGC209300.067.94079646
TATGCCG209450.067.9126348
TCGTATG209900.067.7594945
ATGCCGT210900.067.48077449
CTGCTTG212200.066.9995359
CTCGTAT211650.066.98427644
GCCGTCT212700.066.8640651
TGCCGTC212800.066.8472350
CCGTCTT214350.066.43101552
TGCTTGA214000.066.4177860
TCTCGTA213950.066.24782643
TTAAATC212900.065.9024238
TCTGCTT216150.065.7913558
CTTAAAT214050.065.6991637
ATCTCGT215650.065.69495442
AATCTCG215650.065.4677441
GCTTAAA214900.065.3307436
GCTTGAA218000.065.2954361
CGCTTAA215550.065.1499835