FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3321.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3321.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2409115
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGTCATATCTCGTAT2069098.588589585802255TruSeq Adapter, Index 6 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC213600.067.9671246
TATGCCG213750.067.9086848
GTATGCC214150.067.7776247
TCGTATG214750.067.54864545
ATGCCGT215650.067.2807149
TGCCGTC217650.066.6638350
CTCGTAT217550.066.5183944
GCCGTCT218550.066.3934551
CTGCTTG218600.066.3434859
CCGTCTT219850.066.0340752
TGCTTGA220200.065.8981660
TCTCGTA220450.065.6737443
TATCTCG220750.065.22121441
GTCATAT220100.065.17802437
CATATCT220550.065.0885939
ATCTCGT222750.064.96419542
CGTCATA220350.064.9202236
TCTGCTT223500.064.91894558
GCTTGAA225800.064.2651861
ATATCTC224450.064.0654440
Produced by FastQC (version 0.11.5)