FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052023
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGGAATATCTCGTAT21402920.344517182609128TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG226300.068.45454448
CGTATGC226200.068.4229146
GTATGCC226450.068.3782947
ATGCCGT227100.068.1979949
TCGTATG226750.068.19520645
TGCCGTC227550.068.0202150
GCCGTCT227750.067.9451151
CTGCTTG228200.067.89104559
CTCGTAT227650.067.86410544
CCGTCTT228850.067.6491152
TGCTTGA229200.067.5763560
TCTCGTA228800.067.553643
GCTTGAA230450.067.209861
TCTGCTT230550.067.1958358
ATCTCGT230050.067.1256842
CTTCTGC230750.067.0920956
TATCTCG229450.067.02663441
TTCTGCT232000.066.8211257
ATATCTC230400.066.6192440
AATATCT230150.066.61874439
Produced by FastQC (version 0.11.5)