FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3315.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3315.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463678
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATCTCGTAT27140811.016374704811263TruSeq Adapter, Index 15 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC285100.067.9953647
TATGCCG285300.067.9150248
CGTATGC285400.067.8979946
TCGTATG286150.067.743145
ATGCCGT286600.067.6626849
TGCCGTC288100.067.2510150
CTGCTTG288800.067.1095359
TGCTTGA289550.066.9195360
CTCGTAT289150.066.8829144
GCCGTCT290000.066.835951
CCGTCTT292400.066.33655552
TCTCGTA291800.066.24087543
ATCTCGT293050.065.9914742
TAGCATC289900.065.954738
GCATCTC291650.065.8416140
GCTTGAA294350.065.8282761
TCTGCTT294700.065.792458
CATCTCG294650.065.4193441
AGCATCT293850.065.24412539
ATAGCAT293700.065.06692537