FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3314.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3314.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2286141
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTAAGATCTCGTAT1931768.449872514424964TruSeq Adapter, Index 18 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC209000.067.7900746
GTATGCC209400.067.68024447
TATGCCG209900.067.4871548
TCGTATG209950.067.4637145
ATGCCGT211100.067.0869449
CTCGTAT212050.066.7295744
GCCGTCT212600.066.6179851
CTGCTTG212950.066.5205659
CCTAAGA210050.066.37537436
TGCCGTC213700.066.2572450
CTAAGAT210650.066.2440937
TCCTAAG210300.066.1647635
TGCTTGA214100.066.1069860
TCTCGTA214600.065.855143
CGTCCTA212550.065.7278633
GTCCTAA212750.065.68252634
GATCTCG214100.065.5689641
CCGTCTT216250.065.5421352
ATCTCGT215550.065.5013442
AGATCTC214050.065.4179140
Produced by FastQC (version 0.11.5)