FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3313.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3313.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140539
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCATGATCTCGTAT1757588.210922576042764TruSeq Adapter, Index 6 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC187650.067.6527547
TATGCCG187600.067.6350448
CGTATGC187800.067.6157746
TCGTATG189000.067.16794645
ATGCCGT189500.066.9431349
CTCGTAT190100.066.63042444
TGCCGTC190950.066.437950
GCCGTCT191050.066.38634551
CTGCTTG192300.066.0199259
TGCTTGA193450.065.6590460
TCTCGTA192900.065.6088443
CCGTCTT193500.065.5638952
GATCTCG193900.065.08845541
ATCTCGT194600.065.05215542
GCTTGAA197350.064.43243461
TCTGCTT197200.064.3794758
ATGATCT196400.063.93319339
CATGATC195750.063.9294638
TGATCTC197150.063.74323340
CCTCATG197250.063.20230535
Produced by FastQC (version 0.11.5)