FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3312.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3312.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453286
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATCTCGTAT18927513.02393334828795TruSeq Adapter, Index 15 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG203000.068.207848
GTATGCC203200.068.2072247
CGTATGC203900.067.9902346
ATGCCGT204200.067.76021649
TCGTATG204350.067.7206145
TGCCGTC205150.067.3781850
CTCGTAT205750.067.2257944
TGCTTGA205950.067.17982560
GCCGTCT205950.067.1334651
CTGCTTG206000.067.11484559
CCGTCTT206850.066.8075252
ATCTCGT207150.066.6869842
TCTCGTA208050.066.4489743
GCTTGAA209300.066.13861
TCTGCTT209350.066.04088658
CATCTCG208250.065.99860441
ACCATCT207050.065.9708939
GACCATC206950.065.88438438
CCATCTC209500.065.4332640
CTTCTGC211700.065.2912656
Produced by FastQC (version 0.11.5)