FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3311.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3311.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990137
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAGGACATCTCGTAT28665314.403681756582587TruSeq Adapter, Index 22 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG309900.068.0400248
GTATGCC309950.068.02563547
CGTATGC309800.068.02125546
ATGCCGT310500.067.902449
TCGTATG310700.067.7999845
TGCCGTC311700.067.6556150
CTGCTTG312300.067.5765859
GCCGTCT312350.067.481251
CTCGTAT311650.067.4697944
TGCTTGA314150.067.1958560
TCTCGTA313350.067.0590743
CCGTCTT314900.066.96641552
TCTGCTT316550.066.66930458
GCTTGAA317050.066.603361
ATCTCGT315450.066.60155542
ACATCTC314900.066.27943440
GGACATC314250.066.2416738
CATCTCG317500.066.02656641
GACATCT315850.066.0152639
CTTCTGC321200.065.71503456
Produced by FastQC (version 0.11.5)