FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3310.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3310.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886034
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAATCTCGTAT48983725.971801144624116TruSeq Adapter, Index 12 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG522850.068.8077348
GTATGCC523450.068.7367647
ATGCCGT523250.068.7204649
CGTATGC524000.068.6943746
TCGTATG524200.068.6463245
TGCCGTC525350.068.4475850
CTGCTTG525250.068.4472759
CTCGTAT524350.068.4331144
TGCTTGA525800.068.3968760
GCCGTCT525500.068.39654551
TCTCGTA525650.068.16443
CCGTCTT527800.068.1250252
GCTTGAA528700.068.0415661
TCTGCTT530150.067.8476658
ATCTCGT528200.067.8349242
AATCTCG527250.067.7314741
CTTCTGC531950.067.6162856
GCAATCT526900.067.4292839
CAATCTC529200.067.32840
TTCTGCT534950.067.23411657
Produced by FastQC (version 0.11.5)