FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3309.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3309.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452646
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGTAT33904813.823764212201842TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC366700.068.2723347
TATGCCG366950.068.19720548
TCGTATG367300.068.16894545
CGTATGC367400.068.1408746
ATGCCGT367250.068.0884849
CTCGTAT368350.067.870144
TGCCGTC368700.067.8153650
GCCGTCT369450.067.65874551
CTGCTTG370400.067.47970659
TGCTTGA371550.067.35425660
CGTATCT366200.067.3387739
GTATCTC367350.067.2694940
TCTCGTA371650.067.1921343
CCGTCTT373250.067.00606552
TATCTCG371550.066.702941
ATCTCGT374850.066.5918742
GCTTGAA376250.066.5873161
CCGTATC369700.066.5511538
TCTGCTT375600.066.54819558
TCCGTAT371800.066.2344437
Produced by FastQC (version 0.11.5)