FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3308.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3308.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451779
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGTAT2384059.723755689236265TruSeq Adapter, Index 19 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG259400.068.1540148
GTATGCC259750.068.0756547
CGTATGC259650.068.0182146
TCGTATG260900.067.6895645
ATGCCGT261550.067.5429949
CTCGTAT261450.067.34637544
GCCGTCT263350.066.9951351
TGCCGTC263850.066.9477850
CTGCTTG263600.066.8917559
TCTCGTA264300.066.5658443
TGCTTGA265500.066.49856660
CCGTCTT266800.066.156452
ATCTCGT266750.065.9702742
AATCTCG267250.065.6530841
TCTGCTT269700.065.44504558
GCTTGAA270400.065.3711961
CGAATCT268300.065.07509639
ACCGAAT270600.064.6177837
CCGAATC270650.064.473938
TAACCGA271300.064.4073435
Produced by FastQC (version 0.11.5)