FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3306.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3306.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231667
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATCTCGTAT32525314.57444143772346TruSeq Adapter, Index 20 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG349200.068.40578548
CGTATGC349500.068.35708646
GTATGCC349350.068.3463647
ATGCCGT350300.068.155649
TCGTATG350500.068.1405645
TGCCGTC351900.067.8686550
CTCGTAT351650.067.8197144
CTGCTTG352950.067.7064159
GCCGTCT353650.067.5244251
TGCTTGA354750.067.37958560
TCTCGTA354300.067.3124543
CCGTCTT356450.067.0332952
ATCTCGT355650.066.9471942
TCTGCTT357950.066.8291258
TACATCT353000.066.76565639
GCTTGAA358650.066.6288761
TTACATC353700.066.5657438
CATCTCG355600.066.5447241
GTTACAT355650.066.2499837
CTTCTGC361250.066.2491856
Produced by FastQC (version 0.11.5)