FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3305.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3305.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476911
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCTCGTAT28117119.037775465143124TruSeq Adapter, Index 4 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG302450.068.4316548
GTATGCC302650.068.36098547
CGTATGC302550.068.353546
TCGTATG303250.068.19340545
ATGCCGT303700.068.16382649
TGCCGTC303800.068.1022250
CTCGTAT303350.067.96326444
CTGCTTG304900.067.9001559
TGCTTGA305750.067.7319960
GCCGTCT305550.067.7259251
TCTCGTA304550.067.5805643
CCGTCTT306400.067.5494652
GATCTCG303700.067.3525741
ATCTCGT304950.067.3404442
TCTGCTT307600.067.3246258
GCTTGAA308950.067.0417861
CTTCTGC310350.066.7077756
TTCTGCT310800.066.64046557
TGATCTC306050.066.5701640
CTTGATC304550.066.32838
Produced by FastQC (version 0.11.5)