FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3304.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3304.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943858
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGGTACATCTCGTAT32487916.713103529167253TruSeq Adapter, Index 2 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC348100.068.20103547
TATGCCG348600.068.0966748
CGTATGC348800.068.0724446
ATGCCGT348950.068.00831649
TCGTATG349450.067.9340745
TGCCGTC349950.067.779250
CTCGTAT350450.067.6186344
TGCTTGA350800.067.5663660
GCCGTCT351150.067.5575451
CTGCTTG350950.067.5045359
CCGTCTT353400.067.1472352
GCTTGAA353750.067.0424961
TCTCGTA353600.066.94697643
TCTGCTT354100.066.935458
TACATCT351600.066.6358339
CTTCTGC355900.066.61825656
ATCTCGT355250.066.5769342
ACATCTC353800.066.41930440
CATCTCG355100.066.3192341
GTACATC352650.066.3019138
Produced by FastQC (version 0.11.5)