FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3302.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3302.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649833
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGTAT1666106.287566046615013TruSeq Adapter, Index 16 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC179300.067.577246
TATGCCG179700.067.4098448
GTATGCC180000.067.3351147
ATGCCGT180350.067.1318849
TCGTATG180800.066.9778345
TGCCGTC183250.065.9598750
CTGCTTG183700.065.8413759
CTCGTAT183400.065.83623544
TGCTTGA184400.065.6384760
GCCGTCT184850.065.4280551
TCTCGTA186950.064.5498443
ATCTCGT186900.064.5296642
AATCTCG187050.064.290841
GCTTGAA189500.064.0185161
CCGTCTT189350.063.89160552
CGTTAGA187850.063.38952635
GCGTTAG188350.063.2584234
TCTGCTT191900.063.0644158
TAGAATC189900.062.87767838
CGCGTTA190650.062.7155833
Produced by FastQC (version 0.11.5)