FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3298.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3298.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007343
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAAGATCTCGTAT1736798.652183508249463TruSeq Adapter, Index 1 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG187450.068.042548
GTATGCC187600.067.9677547
CGTATGC188000.067.82313546
ATGCCGT188200.067.7747349
TCGTATG189200.067.4451145
CTCGTAT188800.067.4380144
TGCCGTC190150.067.0629550
CTGCTTG190950.066.8186559
GCCGTCT192250.066.3668251
TGCTTGA192900.066.2108360
TCTCGTA192050.066.2073143
CCGTCTT194600.065.6013452
ATCTCGT193850.065.57612642
GATCTCG193400.065.3832441
AGATCTC193850.065.06734540
TCTGCTT196200.065.0663758
GCTTGAA196300.065.06402661
AAGATCT196300.064.167739
CTTCTGC201600.063.34088556
CCTCAGA200000.063.0075133
Produced by FastQC (version 0.11.5)