FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3295.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3295.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152538
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCTCGTAT21628910.048092066202779TruSeq Adapter, Index 6 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC230250.067.8350647
TATGCCG230300.067.8250648
CGTATGC230400.067.775746
TCGTATG231550.067.45421645
ATGCCGT231650.067.4272149
CTCGTAT232950.066.9301944
TGCCGTC233550.066.88022650
GCCGTCT234400.066.6289651
CTGCTTG235250.066.3876759
TGCTTGA235650.066.334460
TCTCGTA235350.066.17177643
ATCTCGT237400.065.6298542
CCGTCTT238650.065.6037752
GCTTGAA239950.065.17483561
CATCTCG238200.065.14648441
TCTGCTT240350.065.05331458
CCATCTC237650.064.9158840
CTTCTGC243450.064.235456
CACGCAA241750.064.0844431
ACGCAAT241550.064.06653632
Produced by FastQC (version 0.11.5)