FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3286.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3286.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531423
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTACGCAATCTCGTAT26033916.999809980651982TruSeq Adapter, Index 8 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC277750.068.3429647
CGTATGC277650.068.3423646
TATGCCG277650.068.3386848
ATGCCGT278400.068.1531449
TCGTATG278650.068.0845445
TGCCGTC279150.067.9597150
CTCGTAT279200.067.7498644
CTGCTTG279750.067.6946959
GCCGTCT280250.067.6679751
TGCTTGA280650.067.4790660
ATCTCGT280900.067.354542
CCGTCTT281800.067.31039452
GCAATCT279500.067.2639439
TCTCGTA281350.067.24457643
CGCAATC279400.067.2128638
AATCTCG281050.067.1940241
TCTGCTT282800.066.9915558
ACGCAAT281400.066.923937
CAATCTC282200.066.8312140
GCTTGAA283750.066.7810261
Produced by FastQC (version 0.11.5)