FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3285.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3285.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197667
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAGTACATCTCGTAT2186969.951280152998612TruSeq Adapter, Index 7 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC228700.068.2102447
CGTATGC228750.068.1922346
TATGCCG228950.068.1388648
ATGCCGT230150.067.7593449
TCGTATG230450.067.7180245
GCCGTCT231900.067.25259451
TGCCGTC232200.067.1792550
CTCGTAT232050.067.1470644
CTGCTTG232750.067.0024159
TGCTTGA233250.066.85573660
CCGTCTT234700.066.4651852
TCTCGTA235100.066.2461743
TCTGCTT236900.065.7843458
ATCTCGT236800.065.7247542
TACATCT235350.065.6225839
GTACATC235850.065.3795938
CATCTCG237800.065.30118641
GCTTGAA239350.065.22561
ACATCTC238350.065.0153940
AGTACAT238000.064.7610537
Produced by FastQC (version 0.11.5)