FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3283.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3283.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489862
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTACCAATCTCGTAT17345211.642152091938716TruSeq Adapter, Index 15 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG185050.068.1846748
ATGCCGT185450.068.037649
GTATGCC185500.067.9792447
CGTATGC185600.067.9237646
TCGTATG185850.067.7382345
TGCCGTC187150.067.4450650
GCCGTCT187400.067.284951
CTCGTAT186300.067.27402544
CTGCTTG187500.067.1392459
TGCTTGA188450.066.8661160
TCTCGTA188050.066.5921443
CCGTCTT190250.066.3505552
ATCTCGT188850.066.2892842
TCTGCTT190200.066.2413858
GCTTGAA190550.066.2210561
AATCTCG189000.065.9937441
GTACCAA188250.065.9375236
TACCAAT189200.065.83369437
CAATCTC189050.065.8237740
CCAATCT189200.065.679139
Produced by FastQC (version 0.11.5)