FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3280.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3280.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919396
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGATTCATCTCGTAT31841816.58948961027323TruSeq Adapter, Index 10 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC343300.068.3811647
CGTATGC343550.068.331446
TATGCCG344100.068.2544848
ATGCCGT344400.068.13759649
TCGTATG344050.068.1369845
CTCGTAT344850.067.8469844
TGCCGTC346250.067.7854150
GCCGTCT346550.067.6964351
CTGCTTG346900.067.6785759
TGCTTGA348700.067.414360
TCTCGTA347000.067.35599543
CCGTCTT349250.067.1748252
GCTTGAA350800.066.99077661
ATCTCGT349000.066.90983642
TCTGCTT352550.066.5840258
CATCTCG349650.066.5652341
CTTCTGC353200.066.45975556
TTCTGCT356100.065.9202457
TCATCTC353900.065.6996640
ATTCATC353850.065.5704838
Produced by FastQC (version 0.11.5)